Birthe Stüven
(Institute of Innate Immunity, University of Bonn, Germany)
“We measured changes in Ca2+ levels using fluorescent dyes in a reporter HEK cell line to determine the activity of optogenetic tools upon light stimulation. With the pxONE system (red/far red and blue LEDs), we were able to stimulate these tools at specific time points and follow the kinetics of the signal with single cell resolution.”