opto biolabs' pxONE is an LED illumination device designed to combine optogenetic experiments with flow cytometry. It illuminates cell samples homogenously and an attached water bath keeps the temperature stable. Each pxONE is customized towards your specific needs with two build-in wavelenghts between 365-980 nm per device. Software control allows easy selection of light intensity and exposure time.
As a result, optogenetic experiments can be analyzed with over to 30.000 cells/second at single cell resolution. A plethora of established protocols for flow cytometry (calcium flux analysis, apoptosis, multiparameter analysis, cell population analysis etc.) can now easily be adapted to optogenetics.
The pxONE is compatible with BD™ LSR II, BD LSRFortessa™, Cytek™ Aurora, Miltenyi MACSQuant® Analyzer, Bio-Rad ZE5 Cell Analyzer, Beckman Coulter CYTOFlex. Ask us about compatability for other cytometers.
Interested in trying the pxONE yourself? Get a quote here.
opto biolabs' optoMIX is a plug-and-play micromixer specifically designed to prevent settling cells and uneven dispersion during your flow cytometer experiments.
It comes with a set of biocompatible and autoclavable magnets ensuring fast yet gentle stirring of your cells.
Combined with our LED illumination device pxONE, the optoMIX instrument enables optogenetic flow cytometry experiments of unprecedented quality. Inhomogenous illumination and shifting sample distribution are a thing of the past!
The optoMIX (HxWxD 50 mm x 50 mm x 85 mm) is compatible with any opto biolabs pxONE illumination device and fits with most standard flow cytometers (e.g. Miltenyi MACSQuant® Analyzer). Ask us about compatability for other cytometers.
Want to try the optoMIX yourself? Get a quote here.
why optogenetic flow cytometry?
optogenetic cell sorting: become a test pilot!
You don’t want to wait for our next illumination innovation to reach the market? No problem! Apply here to become one of our daring test pilots for our next revolution: Optogenetic Cell Sorting!
Optogenetic cell sorting will allow you to quickly screen and sort optogenetic libraries, separate responding from non-responding cells and use your pure population or single sorted cells for downstream applications.
We are planning to launch our optoSORT illumination device for optogenetic cell sorting in 2022! If you can’t wait until then, we are selecting a few research groups for beta testing of our late prototypes. All you need is an optogenetic cell system that you would like to sort and the OK from your local flow facility. Fill out the application below and we will get back to you!