opto biolabs' pxONE is an LED illumination device designed to combine optogenetic experiments with flow cytometry. It illuminates cell samples homogenously and an attached water bath keeps the temperature stable. Each pxONE is customized towards your specific needs with two build-in wavelenghts between 365-980 nm per device. Software control allows easy selection of light intensity and exposure time.
As a result, optogenetic experiments can be analyzed with over to 30.000 cells/second at single cell resolution. A plethora of established protocols for flow cytometry (calcium flux analysis, apoptosis, multiparameter analysis, cell population analysis etc.) can now easily be adapted to optogenetics.
The pxONE is compatible with BD™ LSR II, BD LSRFortessa™, Cytek™ Aurora, Miltenyi MACSQuant® Analyzer, Bio-Rad ZE5 Cell Analyzer, Beckman Coulter CYTOFlex. Ask us about compatability for other cytometers.
Interested in trying the pxONE yourself? Get a quote here.
opto biolabs' optoMIX is a plug-and-play micromixer specifically designed to prevent settling cells and uneven dispersion during your flow cytometer experiments.
It comes with a set of biocompatible and autoclavable magnets ensuring fast yet gentle stirring of your cells.
Combined with our LED illumination device pxONE, the optoMIX instrument enables optogenetic flow cytometry experiments of unprecedented quality. Inhomogenous illumination and shifting sample distribution are a thing of the past!
The optoMIX (HxWxD 50 mm x 50 mm x 85 mm) is compatible with any opto biolabs pxONE illumination device and fits with most standard flow cytometers (e.g. Miltenyi MACSQuant® Analyzer). Ask us about compatability for other cytometers.
Want to try the optoMIX yourself? Get a quote here.
Haven't heard about optogenetic flow cytometry?
key advantages of optogenetic flow cytometry
applications of optogenetic flow cytometry
need inspiration for your next groundbreaking optogenetic research? How about measuring calcium kinetics with optogenetic flow cytometry (OFC) using the pxONE? Simultaneous illumination and recording ensure a seamless and efficient readout of your data. Learn more about OFC in our white paper.
everything under opto control!
with our user-friendly opto control software you don't have to worry about the reproducibility of your optogenetic experiments any longer. Adjust wavelengths, illumination intensities and exposure times or even create complex and automated illumination patterns with just a few mouse clicks.
this is how easy it is!
See for yourself how quickly you can assemble the pxONE with your cytometer to start your first optogenetic flow cytometry experiment: