The combination of flow cytometry with photoregulated cell samples has never been this easy. Our plug and play pxONE illumination system enables you to analyse your cells with high speed and at single cell resolution.
You can choose wavelengths between 365-980 nm giving you full flexibility for the photostimulation of your cell samples!
Why using the pxONE?
need inspiration for your next groundbreaking optogenetic research? How about measuring calcium kinetics with optogenetic flow cytometry (OFC) using the pxONE? Have a look at this thorough protocol in our publications section. Simultaneous illumination and recording ensure a seamless and efficient readout of your data. Learn more about OFC in our white paper.
with our user-friendly opto control software you don't have to worry about the reproducibility of your optogenetic experiments any longer. Adjust wavelengths, illumination intensities and exposure times or even create complex and automated illumination patterns with just a few mouse clicks.
See for yourself how quickly you can assemble the pxONE with your cytometer to start your first optogenetic flow cytometry experiment!
Over the past years, ever since it was named 'Method of the Year 2010' by the research journal Nature Methods, optogenetics has outgrown its infancy. In the early days used mainly as a neuromodulation method, the application of optogenetic tools at present extends to many different branches of biology. For the most part and despite its many opportunities, current optogenetic in vitro research relies mostly on microscopy, patch clamp-techniques and plate readers, or using self-made and hence often inaccurate illumination devices. In addition, a major drawback of the above-mentioned methods is their inability to perform high throughput experiments at single cell resolution. A powerful tool to overcome these limitations and to revolutionize future optogenetic applications is the combination of flow cytometry with customized LED illumination – OFC. Download our free white paper, written by scientists for scientists.
Have a look at our user stories to get started with your first optogenetic experiments using the pxONE illumination device. Whether you are interested in measuring calcium flux, switching optogenetic tools or determining photopharmacological kinetics, we have you covered.